Transcription Factor 7 Like 2 Gene Polymorphism in Diabetic Patients

Abdalghafour, Hala Saleh; Bakheet, Madeeha Younis; AbdelRazzak, Ghadeer; EL Karn, Ebtsam Farouk; Abd ElMoneim Mohamed, Omnia; Khalifa, Wlaa Anwar; Zaharia Abu Rahma, Mohamed; A. Mohareb, Dina

doi:10.21608/jcmrp.2025.387479.1270

Transcription Factor 7 Like 2 Gene Polymorphism in Diabetic Patients

Document Type : Original Article

Authors

¹ Clinical Pathology Department, Faculty of Medicine, Assiut University Hospital, Assiut, Egypt.

² Medicine Department, Assiut University Hospital, Assiut, Egypt.

³ Department of Tropical Medicine & Gastroenterology, Faculty of Medicine, Assiut University, Assiut, Egypt.

10.21608/jcmrp.2025.387479.1270

Abstract

Abstract: Introduction: Diabetes mellitus (DM) results from insulin resistance, inadequate secretion, and absolute insulin deficiency. DM is associated with macrovascular and microvascular complications. Microvascular complications like diabetic retinopathy, nephropathy, and peripheral neuropathy. The incidence and progression of DM may be influenced by genetic variation. Together with other genes, the transcription factor 7-like 2 (TCF7L2) gene may have had a role in the development of DM. TCF7L2 is an essential constituent of the Wnt signaling pathway and regulates beta cells' insulin release in the pancreas. Patients and Methods: Genotyping of single-nucleotide polymorphism (SNP) rs7903146 of the TCF7L2 gene (using PCR-RFLP) was studied on 54 diabetic patients, 18 prediabetic patients, and 18 apparently healthy individuals as a control group. Results: genotype distributions of TCF7L2 (rs7903146 C/T) SNP in the control group were (CC: 77.8% and CT: 22.2%), respectively, while in the prediabetic group, they were (CC: 55.6% and CT: 44.4%). In the diabetic group, the genotype frequencies were (CC: 27.8%; CT:57.4% and TT:14.8%) respectively. The result of allele distributions of the TCF7L2 (rs7903146 C/T) SNP in the control group was (C allele: 88.9% and T allele: 11.1%), respectively. The prediabetic group was (C allele: 77.8% and T allele: 22.2%) respectively. The diabetic group's frequencies were (C allele: 56.5% and T allele: 43.5%), respectively. The C allele of TCF7L2 (rs7903146 C/T) SNP was predominant in healthy controls. Conclusion: The T allele of TCF7L2 (rs7903146 C/T) SNP was associated with the susceptibility to the development of DM.

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